Evaluation of antimicrobial property of nanochitosan coated orthodontic brackets against <i>Streptococcus mutans</i> and <i>Lactobacillus acidophilus</i> – an <i>in vitro</i> study

Objectives: The objectives of the study were to evaluate and compare antimicrobial property nanochitosan coated uncoated stainless steel (SS) brackets against Streptococcus mutans Lactobacillus acidophilus . Materials Methods: 22 SS orthodontic with bonded crowns extracted premolar teeth prepared for assay S. L. bacterial suspensions incubated in Mueller Hinton broth cultivated Petri dish plates. colonies counted after 1, 6, 12, 24, 72 h intervals using a digital colony counter. Inter intragroup comparisons done independent sample t- test repeated measures ANOVA ( P ≤ 0.05). Results: mean colony-forming units (CFU) showed significant reduction from 1 = 0.000). CFU increase 24 decreased thereafter at 0.000) except between 12 where difference was not statistically 0.837). Mean significantly lesser compared all-time Conclusion: Nanochitosan properties short-term up brackets.